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EzMate Automated Pipetting System

Your Dependable Liquid Handling Partner

EzMate automated pipetting system series is ideal for routine low volume pipetting, and helps eliminate tedious pipetting and human error, which enables more precise and standardized results to improve productivity. With highly-customized adapters and application-oriented software, EzMate can satisfy the various needs of assays, including PCR/qPCR set up, sample normalization, NGS preparation, and reagent kit manufacturing methods.

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Features Applications Specifications Ordering Resources Citations FAQ

Features and Benefits

Simple Operation

● Easy-to-use system setup software without need of additional technical support
● Built-in automatic PCR/qPCR set-up protocols that support USB data transfer

High Flexibility and Capacity

● Single, 4- and 8-channel, 50µl or 200µl automated pipetting modules (APMs)
● 6 interchangeable standard microplate/tip rack adapters and 2 interchangeable reagent vessel adapters
● Active cooling and heating module (ACHM) options for sensitive samples/reagents
● UV lamp and HEPA filter meet the need of high-contamination assays
● Customized adapters support various types of labware

Excellent Serial Dilution Results

Excellent results for qPCR standard curve and replicates.

(Figure) A 10µl synthetic DNA sample is diluted in 30µl water at an 1:4 ratio. Roche® LightCycler® 480 real-time thermal cycler and Finnzyme DyNAmo® Flash SYBR® Green qPCR kit (F-415L) were used.

Better Precision than Manual Pipetting

Better precision and pipetting results than manual pipetting.

(Figure) Target: Human GAPDH (top curve) was amplified in replicates of 4. 18µl (master-mix) and 2µl (cDNA) were pipetted into a 20µl reaction volume. Roche LightCycler 480 real-time thermal cycler and Invitrogen Platinum Taq DNA Polymerase were used.

Performance

Each Automated Pipetting Module (APM) is calibrated using ISO-8655 standards and its high performance shown as the figure.

Applications

A wide variety of liquid handling application support. We also offer customized solutions to suit your experiment need, contact us for further discussion.

 

Typical applications include

 

Specifications

Model

401

601

401s

601s

 

4-position

6-position

4-position&UV/HEPA

6-position&UV/HEPA

Worktable Capacity

SBS Format Area

2 ~ 3

2 ~ 5

2 ~ 3

2 ~ 5

Tip Rack Area

1 ~ 2

1 ~ 4

1 ~ 2

1 ~ 4

Reagent Area

2

2

2

2

Function

Liquid (Sample / Reagent) Transfer (LT)

V

V

V

V

Multiple Dispense (MD)

V

V

V

V

Serial Dilution (SD)

V

V

V

V

Hold (Pause)

V

V

V

V

Mixing (MIX)

V

V

V

V

Loop

V

V

V

V

UV / HEPA

N/A

N/A

V

V

Active Cooling / Heating Module (ACHM)

N/A

N/A

Optional, up to 3 areas

Optional, up to 3 areas

Automated Pipetting Module

Interchangeable 1 / 4 / 8-channel, Maximum volume 50μl / 200μl

Connection

RS-232, USB 3.0 / 2.0

Power Supply

100~240V, 50 / 60 Hz, 100W

Size (W x D x H)

590 x 450 x 470 mm

740 x 450 x 470 mm

660 x 450 x 490 mm

810 x 450 x 490 mm

Weight (N.W.)

25 kg

29 kg

31 kg

35 kg

ACHM Control Box Size (W x D x H)

N/A

N/A

240 x 300 x 230 mm

240 x 300 x 230 mm

ACHM Control Box Weight (N.W.)

N/A

N/A

2 kg

2 kg

Ordering

  • AREZ401-100000
  • 4-position Automatic Pipetting System, 15.6" Notebook Computer and EzStarter control software
  • AREZ601-100000
  • 6-position Automatic Pipetting System, 15.6" Notebook Computer and EzStarter control software
  • EZ4SU00-100000
  • 4-position Automated Pipetting System with UV & HEPA, 15.6" Notebook Computer and EzStarter control software
  • EZ4SUA0-100000
  • 4-position Automated Pipetting System with UV & HEPA and 2-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software
  • EZ4SUAC-100000
  • 4-position Automated Pipetting System with UV & HEPA and 3-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software
  • EZ6SU00-100000
  • 6-position Automated Pipetting System with UV & HEPA, 15.6" Notebook Computer and EzStarter control software
  • EZ6SUA0-100000
  • 6-position Automated Pipetting System with UV & HEPA and 2-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software
  • EZ6SUAC-100000
  • 6-position Automated Pipetting System with UV & HEPA and 3-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software

Resources

Technical article
Detecting COVID-19 Faster and More Efficiently
Application Notes
Validation of gene knockout/knockin in fly models by PCR screening with EzMate 401 Automated Pipetting System
Application Notes
Efficient qPCR Sample and Pool Testing Preparation for COVID-19 Detection Using EzMate Automated Pipetting System
Flyer/Brochure
EzMate Brochure
Application Notes
Accelerate qPCR Setup for COVID-19 Diagnosis on EzMate 601s Automated Pipetting System
Application Notes
Evaluate the Performance of Real-time PCR Instruments by Using EzMate 401 Automated Pipetting System
Application Notes
Consistent serial dilution results using EzMate 401 Automated Pipetting System with 8-channel APM
Application Notes
Sample Normalizations Performed by EzMate 401 Automated Pipetting System
Application Notes
Amplicon-based Library Construction for Next Generation Sequencing Using the EzMate Automated Pipetting System
Application Notes
Fully-Customized Operating Software of EzMate 401 Automated Pipetting System Makes Drug Screening Experiments More Efficient
Manual
EzMate User Manual
Application Notes
LAMP assay for detecting SARS-CoV-2 Using EzMate Automated Pipetting System

Citations

Dec 25, 2022 Immunology & Cell Biology

FLU-LISA (fluorescence-linked immunosorbent assay): high-throughput antibody profiling using antigen microarrays
May 02, 2022 BMC Ecology and Evolution

Environmental DNA analysis confirms extant populations of the cryptic Irwin’s turtle within its historical range
Apr 06, 2022 American Society for Microbiology (ASM) journal

Soil Origin and Plant Genotype Modulate Switchgrass Aboveground Productivity and Root Microbiome Assembly
Sep 22, 2021 npj genomic medicine

Allele-specific RT-PCR for the rapid detection of recurrent SLC12A3 mutations for Gitelman syndrome
View All

FAQ

  • What is the Under Liquid Level function?

    Under Liquid Level is the default setting. When APM aspirates or dispenses, the point of the tip is 1~2mm under the level of the liquid surface.

  • What is the Bottom function?

    The tips will move to a position about 1~2mm above the bottom of the labware when aspirating or dispensing.

  • What is the Top function in dispense?

    The tips will move to the top position of the labware (except for the 384-well microplate) when the APM dispenses.

  • What is the Reverse pipetting function?

    The “Reverse pipetting” function is used to ensure enough liquid is loaded if it is viscous, or after a Multi-Dispense (MD) command. There might not be enough liquid at the last dispense when you use MD command to transfer the liquid. So, it is recommended that "Reverse pipetting" be used to ensure the aspiration of more liquid to increase the accuracy of the last dispense.

  • What is the Blow-Out function?

    After the APM dispenses the set volume of liquid, "Blow-Out" will dispense a little air. This “Blow-Out” function is used to ensure there will be no liquid remaining inside the tip after dispensing. A small amount can be left in the tip especially if the liquid is viscous. In such a situation "Blow-Out" should be used to push the last of the liquid out of the tip.

  • What is the Air Gap function?

    After APM has aspirated the liquid, “Air Gap” will aspirate a little air which will form an air gap in the tip. This “Air Gap” function is used to ensure the liquid will not drip when the APM does a liquid transfer. For example, an alcoholic liquid could easily drip and evaporate. A little air in the tip below the liquid can help to prevent this.

  • Why does the Multiple-Dispense (MD) command have “Loading First Dispense to Source”, “Discard Last Dispense Back to Used Tip Tray” and “Loading Last Dispense Back to Source” options?

    When using the Multi-Dispense (MD) command, the first and last dispense volume is always less accurate, due to the mechanical characteristics of the motor. For applications that require high accuracy, we recommend the selection of “Loading First Dispense to Source”, “Discard Last Dispense Back to Used Tip Tray” and “Loading Last Dispense Back to Source” in Advanced Setting.

  • How many commands can be used in one EzStarter protocol?

    400 commands.

  • Can I use the Import function to load new protocols into EzStarter?

    Yes, EzStarter can import protocols as .csv/.txt format files. Users can set up LT (liquid transfer) and MD (multi-dispense) commands in this import protocol. Please contact us for detailed information.

  • What is the required yearly preventive maintenance?

    - APM calibration needs to be done once a year.
    - the X, Y, Z Rails and the APM driving rod need to be lubricated once a year.
    - the UV lamp needs to be replaced every two years.
    - the HEPA filter needs to be replaced every year or after 1,000 hours of use.

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