TurboQ Real-Time PCR System
Ideal for Multiplex and Routine PCR Applications
TurboQ Real-Time PCR system is designed for multiplex and routine PCR applications with up to 4 targets. Inheriting the proven PCR instrument core competency from Blue-Ray Biotech, TurboQ brings versatile features to help lab researchers in performing diagnostic testing and researches.
Features and Benefits
4 Optical Channels
Detects up to 4 targets in one well
CCD-Based Detection System
The entire plate can be detected at once to eliminate reaction-time differences
Motorized Plate Carrier Tray
Plate in/out with a simple click, enables automation integration in the future
+/- 0.25°C Block Temperature Control Accuracy
Ensures experiment condition stability and eliminates uncertainty
Software with Complete Analysis Functions
Functions include Absolute Quantitation, Relative Quantitation, Allelic Discrimination, Melting Curve. No need to purchase extra analysis modulesSpecifications
Sample Block
- Sample Block
- Fixed 96-well, compatible with regular profile 0.2 ml PCR tube, strip, and semi-skirted 96-well plate
- Sample Volume
- 10 - 100 µl
- Plate Loading
- Motorized plate carrier tray
Block Temperature
- Block Temperature Range
- 25 - 99 °C
- Peak Block Ramp Rate
- 2.5 °C/sec
- Temperature Accuracy
- ± 0.25 °C (35 °C – 95 °C) of set point/ display temperature / Measured at 3 minutes after clock start.
- Temperature Uniformity Across Block
- ± 0.5 °C (30 seconds after clock start)
Gradient Temperature
- Gradient Temperature Range
- 60 - 99 °C
- Gradient Temperature Difference
- Max. span 25 °C
Heater Lid
- Heating Lid
- 105°C
Optics
- Excitation Source
- Tungsten-halogen lamp
- Detection Module
- CCD camera
- Detection Channel
- 4 channels (Ch1: FAM, SYBR Green, Ch2: HEX, VIC, JOE, Ch3: ROX, Texas Red, Ch4:Cy5)
Environment
- Operating Temperature
- 15 – 30 °C
- Operating Humidity
- RH: 85% or less
- Storage Temperature
- -10 - 60 °C
General
- PC Operation System
- Windows 7 or higher
- Footprint Dimensions (H x W x D)
- 567 mm x 354 mm x 455 mm
- Weight
- 35 kg
- Power Supply
- 110/230 V, frequency 50/60 Hz, 1500W
- Fuse
- 1 x fuse, 10A for 110VAC power / 5A for 220VAC power
* Specifications are subject to change without prior notice.
Ordering
- TCRT-9614
- Real-Time PCR TurboQ 110V Ch1,2,4,5
- TCRT-9624
- Real-Time PCR TurboQ 220V Ch1,2,4,5
- TCRT-C001
- TurboQ 4ch Calibration Kit
FAQ
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How to clean the wells and lid heater of the TurboQ?
Periodically wipe it, clean of dust, and other residue that comes from normal operation of the unit. Use a soft, lint-free cloth and deionized water. Air vents should be vacuumed to remove dust.
Before cleaning the lid heater, make sure the TurboQ is turned off, unplugged, and cooled down. Use a mild detergent to clean debris from the lid. A Kimwipe™ dipped in 70% ethanol will help remove residue from the seal. Make sure the lid is dry before plugging in the power cable.
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What are the differences between absolute and relative quantification?
-Absolute quantification involves the determination of the number of molecules or the copy number in the sample. It applies to the concentration of a standard sample for making a standard curve, which allows a comparison to be made with unknown samples for an estimation of their concentration.
-Relative quantification involves the comparison of differences between unknown samples and a reference gene. The test will indicate how many folds more or less it has compared to the reference gene. This is commonly used in treatment tests, to compare control genes with treated genes.
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Does “melt curve” different from” high resolution melt curve”?
Yes, see below:
-melt curve: the melting curve traces progress as the double stranded DNA slowly degrades to single strands with increasing temperature. It can be used to determine the presence of nonspecific products after PCR cycling.
-high resolution melt curve: a high-resolution melt curve provides a quantitative analysis of the melting of double stranded DNA after PCR cycling. A high resolution melt curve can read the sample in 0.1°C increments, which means it can differentiate samples very precisely and can show differences caused by a single base pair. Only the qPCR with great thermal stability and sensitivity which has HRM-dedicated software can prepare a “high resolution melt curve”. It can be used for the analysis of SNP genotyping, gene mutation, gene methylation, microRNA… etc.
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How often should optical and dye calibration be needed for the TurboQ?
-Optical calibration: Optical calibration is only needed at first-time installation, or after the equipment has been moved to another site or position and has been reinstalled.
-Dye calibration: We recommend annual dye calibration.
However, dye calibration should be done if experimental results become abnormal.
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How do I decide if Ct based or RFU based analysis is the most suitable method for my Allelic Discrimination experiments?
Ct based analysis is most commonly used, but if the efficiency of your samples is low and it is difficult to reach a plateau, it may be impossible to get a correct Ct value. In such a situation, RFU based analysis which can discriminate different genes will get better results.
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Will a “different ramping rate” change my results?
Yes, some enzymes are very sensitive to small changes in temperature. So, we recommend that the ramping rate be set according to the user manual recommendation for a particular enzyme.
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What kinds of fluorescent can I detect in TurboQ?
There are four detection channels in TurboQ, and the detected fluorescence of each channel is as below.
-Ch1: FAM, SYBR Green
-Ch2: HEX, VIC, JOE
-Ch3: ROX, Texas Red
-Ch4: Cy5
