Easy to Control
The sensitive 7” capacitive touchscreen enables easy operation even with laboratory gloves.
TurboCycler 2 Thermal Cycler
Enhancing PCR Efficiency and Accuracy
TurboCycler 2 thermal cycler is designed specifically to enhance PCR efficiency and accuracy. It is equipped with a 7” sensitive touchscreen and a friendly graphic user interface, which makes operation highly intuitive. With flexible ramp rate and gradient temperature control, TurboCycler 2 greatly increases PCR accurate optimization. The Wi-Fi function enables you to monitor the PCR run status anytime remotely with walk-away convenience.
Features and Benefits
Outstanding Performance
Flexible Ramp Rate Control
from 0.1 - 5.5 ℃/sec to meet the need for the CRISPR related assays .
Fully Adjustable Lid Temperature
can be set between 35 and 120 °C for virtually any type of experiment including NGS pre-treatment.
Gradient Optimization
range from 1 to 30 °C enables optimal experimental conditions in a single PCR run.
Friendly User Interface
The graphic interface is easy to use, making the adjustment of experiment parameters such as temperature, time and cycle quick and simple.
Efficient Remote Monitoring
Monitor your PCR status remotely anytime on your mobile device via the free TurboApp.
TurboCycler Model Comparison
Specifications
Sample Block
- 96 Well, Gradient Block
- Compatible with regular profile or low profile 0.2 ml PCR tube, strip, non-skirted, semi-skirted and full-skirted 96-well plate
Block Temperature
- Block Temperature Range
- 4.0 - 100 °C
- Max. Heating Rate
- 5.5 °C/sec
- Max. Cooling Rate
- 3.3 °C/sec
- Temperature Accuracy
- +/- 0.3 °C
- Temperature Uniformity Across Block
- +/- 0.3 °C
- Adjustable Ramp Rate
- 0.1 - 5.5 °C/sec
Gradient Temperature
- Gradient Direction
- Horizontal across the block
- Gradient Temperature Range
- 30 - 100 °C
- Gradient Temperature Difference
- Max. span 30 °C
Heater Lid
- Temperature Setting Range
- 35 - 120 °C or off
- Temperature Accuracy
- +/- 1.0 °C
Software
- Portability of Protocols
- Save and transfer to computer or TurboCycler 2 via USB flash drive
- Stored Program No.
- > 4000 sets
- Registered User Folder No.
- 100 sets
- User Folder Password Protection
- Yes
- Run Status Report
- Yes, HTML output and transfer via USB flash drive
- Real-time Temp. Profile Export
- Yes, CSV output and transfer via USB flash drive
- Tools
- Tm calculator, Copy number convertor, Master mix preparation wizard
General
- Display
- 7” color LCD with capacitive touch panel
- Data Port
- 1 USB Type-A front port for USB flash drive
- Heated Lid
- 35 - 120 °C or off
- Auto Restart after Power Outage
- Yes
- Remote Monitoring via Wi-Fi
- Optional
- Footprint Dimensions (H x W x D)
- 225 mm x 245 mm x 415 mm
- Weight
- 9.5 kg
- Power Supply
- AC 100-240 V, 50/60 Hz, 750 W
- Certification
- CE, RoHS
* Specifications are subject to change without prior notice.
Ordering
- TCST-9612
- Gradient TurboCycler 2 with 96-well Sample Block (110V)
- TCST-9622
- Gradient TurboCycler 2 with 96-well Sample Block (220V)
- TCLT-a001
- Wi-Fi Upgrade Module
- TCLT-a002
- 5.2 mm Compression Mat x 10 pcs
- TCLT-a003
- 1.0 mm Compression Mat x 10 pcs
Resources
Citations
FAQ
-
Compressor Mat and Low-profile PCR vessel?
The low-profile PCR vessel design reduces the overhead space above the reaction solution inside the vessel.
During thermal cycling, water in the solutions evaporates and fills the overhead space. This causes a concentration increase in the reacting solution.
The smaller the overhead space, the smaller the amount of evaporated water and concentration change in the solution. This is especially helpful when low volume reaction mixtures are being used.
In general, the low-profile PCR vessels are 5 mm shorter in height than the regular PCR vessels.
Use the compressor mat and set the lid heat to 120°C to maintain the pressure in the vessels. -
What is the function of the lid? What is the effect on evaporation? Does the lid temperature cycle during experiments? Do the competitive units do the same? What is the temperature of the lid at the 4°C step?
Lid heating is used to avoid severe condensation in the PCR tubes during the reaction.
The amount of evaporation is related to the sealing of the PCR vessels. A firm pressure on the lid will help maintain the integrity of the seal and prevent evaporation.
The Lid heating does not cycle and the competitive units do not cycle either.
When the block temperature drops below 30°C, lid heating is turned off and the temperature of lid decreases gradually. Competitive units also do this. -
Condensation vs Evaporation
Condensation inside the tube is caused by the temperature difference between the tube and the cycler. The tube temperature might remain at 4°C for a long time at the end of a run. This happens with all cyclers. It is like a bottle of water being put into a refrigerator; water always condenses in the neck.
The amount of water lost to the environment from a reaction vessel by evaporation depends on the integrity of the seal.
If the seal or the vessel cannot withstand the temperature cycling during the run, there may be deformation. This deformation may be serious enough to tear off the seal. Plates and seals of poor quality may deform enough to disrupt the seal. Low quality consumables should be avoided. It is essential that the plates and seals remain undeformed for the entire run.
-
When a run is started, the block holds for several minutes before it starts to heat up. Why is this?
The lid of the cycler needs to be heated to the proper temperature before the block starts to heat.
The delay will depend on the lid temperature setting, the room temperature, and how the compression mat has been used. Heating of the lid usually takes between 2 to 8 minutes.
-
When the power comes back after a power failure, do the TurboCyclers (Lite/2) start running again?
When the power comes back on again after a “power-failure-during-a-run” event, the unit will start up and continue where it had stopped and run until the end of the protocol. This will happen as long as the power switch has not been touched in the meantime.
Also, if the power stays off for a long time, there will be a short delay (after the power comes back on) before the unit starts again. This is because it might take a minute or two for the lid to get back up to the right temperature.
After the lid module reaches its setting temperature, the protocol will be continued. This “Auto-restart after power outage” function has been specifically designed to protect the PCR experiment. -
Can TurboCycler 2 and TurboCycler Lite run overnight?
Yes! But we do not recommend this be done very often.
Running your cycler overnight at 4°C can potentially reduce the life cycle of the instrument. In most applications, 16°C instead of 4°C for overnight storage can protect your DNA sample very well.
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Is the ramping rate of TurboCycler 2 adjustable?
Yes! The ramping rate of TurboCycler 2 can be adjusted from 0.1°C to 5.5°C.
-
What is the temperature range of the lid of TurboCycler 2 and TurboCycler Lite?
There are three different temperature settings for the lid:
1. 120°C (for use with low-profile vessel + compression mat), with an extra 3 minutes of pre-heat
2. 105°C
3. Heater off
-
Are the blocks of the TurboCycler 2 and TurboCycler Lite interchangeable?
There is only one block configuration for the TurboCycler Lite and TurboCycler 2 so far. They are not interchangeable.
However, if the block needs to be serviced it can removed and sent back to our factory for service.
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How many protocols and history can be stored in TurboCycler 2? What will happen if my data exceeds the limit?
The storage limits are:
-Protocol: >4000 sets
-History: 50 sets
-User folder: 100 sets
When the storage of protocols has reached the limit, a message will remind the user to delete some protocols. The system will not overwrite existing protocols.
However, when history storage reaches the limit, new histories will over-write the oldest. But the user can set a reminder that shows when history storage gets close to the limit.
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What are block and sample mode for TurboCycler 2?
-Block mode: Enables system operation with maximum temperature control based on the actual block temperature regardless of the sample temperature. This mode is designed to mimic the control algorithm used in most early model thermal cyclers.
-Sample mode: Enables system operation with simulated temperature control. Users are required to input the sample volume: 5-100μl. In this mode, the temperature control algorithm will estimate the sample temperature based on the block temperature and sample volume. This is the mode recommended for normal operation.
