Accelerate qPCR Setup for COVID-19 Diagnosis on EzMate 601s Automated Pipetting System

Benefits

 

  • Increase speed and precision of preparing qPCR mastermix on EzMate 601s Automated Pipetting System for COVID-19 diagnostic tests

  • Customized method and labware layouts based on your application need

 

Customer's Request

 

During the pandemic of SARS-CoV-2, one of our customer is preparing the automation solutions for the diagnostic laboratories, including a viral RNA isolation and preparation of COVID-19 diagnostic tests based on Real-Time PCR. The company asked our help to prepare a complete methods and layout for qPCR Setup of their tests. The following are our suggestions.

 

Our Suggestions

 

1.Using EzMate 601s Automated Pipetting Sytem to prepare the Master Mix:

 

If the user would like to use liguid handling robot to prepare the Maser Mix (19 ul Super Mix + 1ul Enzyme Mix), the EzMate 601s Automated Pipetting System can be implemented to set up maximum 50 tests per run. (2 pieces of the Real Time RT-PCR Kit)

Accelerate_qPCR_Setup_for_SARS-CoV-2 Diagnosis_with_EzMate_601sAccelerate_qPCR_Setup_for_SARS-CoV-2 Diagnosis_with_EzMate_601s_02

-Blue color: sample tubes (1.5/2ml tube)

-Purple color (1) (4):  Super Mix

-Purple color (2) (5): Enzyme Mix

-Purple color (3) (6): Tube for storing Master Mix (Super Mix + Enzyme Mix)

-Red color : PCR tube (8 or 12 strip tube)

-Green color: Tips

 

2.Prepare the Maser Mix manually :

 

If the user prefers preparing the Maser Mix (19 ul Super Mix + 1ul Enzyme Mix) manually, the EzMate 601s Automated Pipetting System can set up maximum 75 tests per run (3 pieces of the Real Time RT-PCR Kit)

 

DOWNLOAD APPLICATION NOTE

 

PCR/qPCR Set Up Related Applications

 


 

Learn More About EzMate Automated Pipetting System

 

We offer customized solutions to suit your experiment need, contact us for further discussion.