Simple Operation
● Easy-to-use system setup software without need of additional technical support
● Built-in automatic PCR/qPCR set-up protocols that support USB data transfer
EzMate Automated Pipetting System
Your Dependable Liquid Handling Partner
EzMate automated pipetting system series is ideal for routine low volume pipetting, and helps eliminate tedious pipetting and human error, which enables more precise and standardized results to improve productivity. With highly-customized adapters and application-oriented software, EzMate can satisfy the various needs of assays, including PCR/qPCR set up, sample normalization, NGS preparation, and reagent kit manufacturing methods.
Features and Benefits
High Flexibility and Capacity
● Single, 4- and 8-channel, 50µl or 200µl automated pipetting modules (APMs)
● 6 interchangeable standard microplate/tip rack adapters and 2 interchangeable reagent vessel adapters
● Active cooling and heating module (ACHM) options for sensitive samples/reagents
● UV lamp and HEPA filter meet the need of high-contamination assays
● Customized adapters support various types of labware
Excellent Serial Dilution Results
Excellent results for qPCR standard curve and replicates.
(Figure) A 10µl synthetic DNA sample is diluted in 30µl water at an 1:4 ratio. Roche® LightCycler® 480 real-time thermal cycler and Finnzyme DyNAmo® Flash SYBR® Green qPCR kit (F-415L) were used.
Better Precision than Manual Pipetting
Better precision and pipetting results than manual pipetting.
(Figure) Target: Human GAPDH (top curve) was amplified in replicates of 4. 18µl (master-mix) and 2µl (cDNA) were pipetted into a 20µl reaction volume. Roche LightCycler 480 real-time thermal cycler and Invitrogen Platinum Taq DNA Polymerase were used.
Performance
Each Automated Pipetting Module (APM) is calibrated using ISO-8655 standards and its high performance shown as the figure.
Applications
A wide variety of liquid handling application support. We also offer customized solutions to suit your experiment need, contact us for further discussion.
Typical applications include
- Sample Preparation
- PCR/qPCR set up
- NGS Library Preparation
- Reagent kit manufacturing
- Cell-based assay
Specifications
|
Model |
401 |
601 |
401s |
601s |
|
|
4-position |
6-position |
4-position&UV/HEPA |
6-position&UV/HEPA |
|
Worktable Capacity |
||||
|
SBS Format Area |
2 ~ 3 |
2 ~ 5 |
2 ~ 3 |
2 ~ 5 |
|
Tip Rack Area |
1 ~ 2 |
1 ~ 4 |
1 ~ 2 |
1 ~ 4 |
|
Reagent Area |
2 |
2 |
2 |
2 |
|
Function |
||||
|
Liquid (Sample / Reagent) Transfer (LT) |
V |
V |
V |
V |
|
Multiple Dispense (MD) |
V |
V |
V |
V |
|
Serial Dilution (SD) |
V |
V |
V |
V |
|
Hold (Pause) |
V |
V |
V |
V |
|
Mixing (MIX) |
V |
V |
V |
V |
|
Loop |
V |
V |
V |
V |
|
UV / HEPA |
N/A |
N/A |
V |
V |
|
Active Cooling / Heating Module (ACHM) |
N/A |
N/A |
Optional, up to 3 areas |
Optional, up to 3 areas |
|
Automated Pipetting Module |
Interchangeable 1 / 4 / 8-channel, Maximum volume 50μl / 200μl |
|||
|
Connection |
RS-232, USB 3.0 / 2.0 |
|||
|
Power Supply |
100~240V, 50 / 60 Hz, 100W |
|||
|
Size (W x D x H) |
590 x 450 x 470 mm |
740 x 450 x 470 mm |
660 x 450 x 490 mm |
810 x 450 x 490 mm |
|
Weight (N.W.) |
25 kg |
29 kg |
31 kg |
35 kg |
|
ACHM Control Box Size (W x D x H) |
N/A |
N/A |
240 x 300 x 230 mm |
240 x 300 x 230 mm |
|
ACHM Control Box Weight (N.W.) |
N/A |
N/A |
2 kg |
2 kg |
Ordering
- AREZ401-100000
- 4-position Automatic Pipetting System, 15.6" Notebook Computer and EzStarter control software
- AREZ601-100000
- 6-position Automatic Pipetting System, 15.6" Notebook Computer and EzStarter control software
- EZ4SU00-100000
- 4-position Automated Pipetting System with UV & HEPA, 15.6" Notebook Computer and EzStarter control software
- EZ4SUA0-100000
- 4-position Automated Pipetting System with UV & HEPA and 2-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software
- EZ4SUAC-100000
- 4-position Automated Pipetting System with UV & HEPA and 3-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software
- EZ6SU00-100000
- 6-position Automated Pipetting System with UV & HEPA, 15.6" Notebook Computer and EzStarter control software
- EZ6SUA0-100000
- 6-position Automated Pipetting System with UV & HEPA and 2-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software
- EZ6SUAC-100000
- 6-position Automated Pipetting System with UV & HEPA and 3-position Active Cooling / Heating module, 15.6" Notebook Computer and EzStarter control software
Resources
Technical article
Detecting COVID-19 Faster and More Efficiently
Application Notes
Validation of gene knockout/knockin in fly models by PCR screening with EzMate 401 Automated Pipetting System
Application Notes
Efficient qPCR Sample and Pool Testing Preparation for COVID-19 Detection Using EzMate Automated Pipetting System
Flyer/Brochure
EzMate Brochure
Application Notes
Accelerate qPCR Setup for COVID-19 Diagnosis on EzMate 601s Automated Pipetting System
Application Notes
Evaluate the Performance of Real-time PCR Instruments by Using EzMate 401 Automated Pipetting System
Application Notes
Consistent serial dilution results using EzMate 401 Automated Pipetting System with 8-channel APM
Application Notes
Sample Normalizations Performed by EzMate 401 Automated Pipetting System
Application Notes
Amplicon-based Library Construction for Next Generation Sequencing Using the EzMate Automated Pipetting System
Application Notes
Fully-Customized Operating Software of EzMate 401 Automated Pipetting System Makes Drug Screening Experiments More Efficient
Manual
EzMate User Manual
Application Notes
LAMP assay for detecting SARS-CoV-2 Using EzMate Automated Pipetting System
Citations
FAQ
-
What is the Under Liquid Level function?
Under Liquid Level is the default setting. When APM aspirates or dispenses, the point of the tip is 1~2mm under the level of the liquid surface.
-
What is the Bottom function?
The tips will move to a position about 1~2mm above the bottom of the labware when aspirating or dispensing.
-
What is the Top function in dispense?
The tips will move to the top position of the labware (except for the 384-well microplate) when the APM dispenses.
-
What is the Reverse pipetting function?
The “Reverse pipetting” function is used to ensure enough liquid is loaded if it is viscous, or after a Multi-Dispense (MD) command. There might not be enough liquid at the last dispense when you use MD command to transfer the liquid. So, it is recommended that "Reverse pipetting" be used to ensure the aspiration of more liquid to increase the accuracy of the last dispense.
-
What is the Blow-Out function?
After the APM dispenses the set volume of liquid, "Blow-Out" will dispense a little air. This “Blow-Out” function is used to ensure there will be no liquid remaining inside the tip after dispensing. A small amount can be left in the tip especially if the liquid is viscous. In such a situation "Blow-Out" should be used to push the last of the liquid out of the tip.
-
What is the Air Gap function?
After APM has aspirated the liquid, “Air Gap” will aspirate a little air which will form an air gap in the tip. This “Air Gap” function is used to ensure the liquid will not drip when the APM does a liquid transfer. For example, an alcoholic liquid could easily drip and evaporate. A little air in the tip below the liquid can help to prevent this.
-
Why does the Multiple-Dispense (MD) command have “Loading First Dispense to Source”, “Discard Last Dispense Back to Used Tip Tray” and “Loading Last Dispense Back to Source” options?
When using the Multi-Dispense (MD) command, the first and last dispense volume is always less accurate, due to the mechanical characteristics of the motor. For applications that require high accuracy, we recommend the selection of “Loading First Dispense to Source”, “Discard Last Dispense Back to Used Tip Tray” and “Loading Last Dispense Back to Source” in Advanced Setting.
-
How many commands can be used in one EzStarter protocol?
400 commands.
-
Can I use the Import function to load new protocols into EzStarter?
Yes, EzStarter can import protocols as .csv/.txt format files. Users can set up LT (liquid transfer) and MD (multi-dispense) commands in this import protocol. Please contact us for detailed information.
-
What is the required yearly preventive maintenance?
- APM calibration needs to be done once a year.
- the X, Y, Z Rails and the APM driving rod need to be lubricated once a year.
- the UV lamp needs to be replaced every two years.
- the HEPA filter needs to be replaced every year or after 1,000 hours of use.