EzQuant Quantification Assay Kits

EzQuant quantification assays are your trusted choice for dsDNA HS, ssDNA, and RNA HS detection. These assays utilize a highly specific fluorescent dye that binds precisely to the target molecule. This allows accurate quantification down to 10 pg/µl, even in the presence of contaminants or degraded DNA/RNA. EzQuant quantification assays only require a 1 µl sample and a quick 2-minute incubation, to significantly enhance the efficiency of your quantification processes.

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Features and Benefits

Blue-Ray Biotech EzCube Fluorometer range plot

 

 

Figure 1. Quantitative initial sample concentration ranges (variable sample volume 1 - 20 µl) for EzQuant quantification assays and UV absorbance measurements.UV absorbance measurement can quantify high concentration samples, but it is not selective for DNA and RNA.

 

-1X dsDNA HS (0.01 - 100 ng/µl)
-ssDNA (0.05 - 200 ng/µl)
-RNA HS (0.25 - 100 ng/µl)
-UV (2 - 20,000 ng/µl)

EzQuant 1X dsDNA HS Quantification Kit

EzQuant 1X dsDNA HS Quantification Kit contains a highly specific fluorescent dye that is designed for samples with low concentrations of dsDNA, and has good tolerance to contaminants. It provides ready-to-use working solutions and standards that you only need to prepare 1-20 µl of sample and perform measurements with the EzCube Fluorometer, and you can easily and accurately quantify DNA sample concentrations ranging from 10pg/μl to 100 ng/μl.

 

Blue-Ray Biotech EzQuant Quantification Kit 1X dsDNA HS Sensitivity

Figure 2. DNA sensitivity of the EzQuant 1X dsDNA HS quantification assay.

Blue-Ray Biotech EzQuant  Quantification Kit 1X dsDNA HS Sensitivity
Blue-Ray Biotech EzQuant  Quantification Kit 1X dsDNA HS Linearity

Figure 3. Linearity between measured and prepared dsDNA concentrations for EzQuant and Qubit™ assays.

Blue-Ray Biotech EzQuant  Quantification Kit 1X dsDNA HS Linearity

 

EzQuant ssDNA Quantification Kit

EzQuant ssDNA Quantitative Kit is for ssDNA or oligonucleotides. It enables quick and accurate quantification of low-concentration samples from 50 pg/µl to 200 ng/µl when using with EzCube fluorometer. It does not specifically recognize ssDNA but can also bind to dsDNA and RNA. However, assay results will not be affected by common contaminants; such as proteins, salts, and detergents.

 

Blue-Ray Biotech EzQuant Quantification Kit ssDNA Linearity

Figure 4. Linearity between measured and prepared ssDNA concentrations for EzQuant and Qubit™ assays.

Blue-Ray Biotech EzQuant Quantification Kit ssDNA Linearity

EzQuant RNA HS Quantification Kit

EzQuant RNA HS Quantification Kit contains a highly specific fluorescent dye designed for samples with low concentrations of RNA and it is well tolerant to contaminants. Enabling the quick and accurate of low-concentration RNA samples ranging from 0.25 to 100 ng/µl when using with EzCube fluorometer.

 

Blue-Ray Biotech EzQuant Quantification Kit RNA HS sensitivity

Figure 5. RNA sensitivity of EzQuant RNA HS quantification assay.

Blue-Ray Biotech EzQuant Quantification Kit RNA HS Linearity

Figure 6. Linearity between measured and prepared RNA concentrations for EzQuant and Qubit™ assays.

Note:

Qubit™ is a registered trademark of Thermo Fisher Scientific and its subsidiaries. It is used here for identification and reference purposes only.

Blue-Ray Biotech, its products and this document are not endorsed, authorized, or affiliated in any way with Thermo Fisher Scientific.

Ordering

  • BRFP-0200
  • EzCube Fluorometer (Blue & Red)
  • BRFP-0300
  • EzCube Fluorometer (Blue, Red & Green)
  • T00-FPBR00-00
  • EzQuant 1X dsDNA HS Quantification Kit (100 Assays)
  • T00-FPBR01-00
  • EzQuant ssDNA Quantification Kit (100 Assays)
  • T00-FPBR02-00
  • EzQuant RNA HS Quantification Kit (100 Assays)
  • T00-FPBR03-00
  • 0.5 ml Thin Wall PCR Tube

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