Suspended 3D Tumorsphere Chip Using EzScope 101 Live Cell Imaging System

Application Note of EzScope 101 Live Cell Imaging System
Author: Hao-Hsiang Hsu

 

Objective


Use a self-made suspended tumorsphere chip to facilitate the formation of a 3D sphere which simulates tumor formation and may serve as a high-throughput drug screening system.

 

Process

 

A tumorsphere chip was produced using a PMMA plate and laser cutter. Tumor cells were introduced and washed. The cells precipitated in a 1-mm pore at the bottom and would proliferate into a sphere in approximately 1-2 days.

 

Result

 

Cells Just Introduced:

cells just introduced

12 hours:

12hr 3d tumorsphere chip

24 hours:

24hr 3d tumorsphere chip

The time-lapse video 

10X objective lens
Capturing interval: 2 min
Replay frame rate: 10 fps

 

Original State of Cells: Human Ovarian Cancer Cell (HM-4)

human ovarian cancer cell

After the Experiment:

24hr 3d tumorsphere chip

 

Future Research Studies

 

Following tumorsphere formation, drugs can be directly introduced through the chip to observe cell response, serving as a drug screening platform, or a second cell can be introduced to envelop the tumorsphere, such as introducing brain epithelial cells to envelop a brain tumorsphere to simulate the blood-brain barrier.

 

Conclusions

 

EzScope 101 realizes prolonged live cell observation that was previously unavailable, allows observation within an incubator, and the built-in software provides sketching and calculation to save post-production time, making it a really convenient device.

 

Learn More about EzScope 101 Live Cell Imaging System