When the power comes back on again after a “power-failure-during-a-run” event, the unit will start up and continue where it had stopped and run until the end of the protocol. This will happen as long as the power switch has not been touched in the meantime.
Also, if the power stays off for a long time, there will be a short delay (after the power comes back on) before the unit starts again. This is because it might take a minute or two for the lid to get back up to the right temperature.
After the lid module reaches its setting temperature, the protocol will be continued. This “auto-restart after power outage” function has been specifically designed to protect the PCR experiment.
Yes! But we do not recommend this be done very often.
Running your cycler overnight at 4°C can potentially reduce the life cycle of the instrument. In most applications, 16°C instead of 4°C for overnight storage can protect your DNA sample very well.
Yes! The ramping rate of TurboCycler 3 can be adjusted from 0.1°C to 5.0°C.
There are four different temperature settings for the lid:
1. 130°C (for use with low-profile vessels + compression mat), with an extra 3 minutes of pre-heat
2. 105°C
3. Heater off
4. Any temperature between 35°C - 130°C
The three blocks of TurboCycler 3 are interchangeable, which indicates you can switch blocks to random positions. Moreover, TurboCycler 3 can normally operate with 1 or 2 block(s) absent. If any block requires maintenance, just send it to your local Blue-Ray distributor.
The storage limits are:
- Protocol: >4000 sets
- History: 50 sets
- User folder: 100 sets
When the storage of protocols has reached the limit, a message will remind the user to delete some protocols. The system will not overwrite existing protocols.
However, when history storage reaches its limit, new histories will over-write the oldest. However, the user can set a reminder that shows when history storage gets close to the limit.
Each heating block has its individual heating element, heat sink, electric fan, ventilation channel, etc., so they will not interact with each other.
- Block mode: Enables system operation with maximum temperature control based on the actual block temperature regardless of the sample temperature. This mode is designed to mimic the control algorithm used in most early model thermal cyclers.
- Sample mode: Enables system operation with simulated temperature control. Users are required to input the sample volume: 5-100μl. In this mode, the temperature control algorithm will estimate the sample temperature based on the block temperature and sample volume. This is the mode recommended for normal operation.
The LED light intensity is preset and cannot be manually adjusted.
Please note that EzCube only supports USB drives formatted in FAT or FAT32. If the USB drive is not in the correct format, the device may not recognize it. Make sure to check and format your USB drive accordingly before use.