Performance Validation of EzQuant Quantification Assay Kits on EzCube, Qubit™ 4, and DS-11 FX+ Fluorometers
2026-03-27
Ensuring High Consistency and Precision Across Industry-Leading Platforms
Executive Summary
Accurate nucleic acid quantification is fundamental for the success of downstream genomic applications. This application note evaluates the performance of EzQuant Quantification Assay Kits across three leading fluorometric platforms: Blue-Ray Biotech EzCube, Thermo Fisher® Qubit™ 4, and DeNovix® DS-11 FX+.
By comparing accuracy across a range of concentrations and sample types, we demonstrate that EzQuant kits provide high sensitivity and cross-platform consistency for dsDNA, RNA, and ssDNA. Notably, the EzCube fluorometer delivers performance equivalent to the Qubit™ 4, providing industry-standard precision across essential laboratory workflows.
Table of contents
- Introduction
- Materials and Methods
- Comparative Performance Data
- Conclusion
Table of contents
- Introduction
- Materials and Methods
- Comparative Performance Data
- Conclusion
Introduction
The EzQuant Quantification Assay Kits utilize a highly specific fluorescent dye to bind precisely to target molecules at low concentrations, ensuring high sensitivity and minimal interference.
The suite includes specialized kits designed for specific detection requirements:
- 1X dsDNA HS: Detects initial sample concentrations from 10 pg/µl to 100 ng/µl.
- RNA HS: Detects total RNA initial sample concentrations from 0.25 to 100 ng/µl.
- ssDNA: Detects ssDNA initial sample concentrations from 0.05 to 200 ng/µl.
Materials and Methods
To validate cross-platform consistency, five samples were tested for each kit:
- Standard-Derived Samples: Three samples were prepared by diluting the kit's Standard 2.
- Extracted Samples: Two samples were nucleic acid extracts obtained via silica membrane-based extraction to simulate real-world laboratory conditions.
All measurements were conducted on the EzCube, Thermo Fisher® Qubit™ 4, and DeNovix® DS-11 FX+. Results are reported in ng/µl.
Comparative Performance Data
Table 1. EzQuant 1X dsDNA HS Kit Performance
| Sample / Expected Conc. | EzCube | Qubit™ 4 | DS-11 FX+ |
|---|---|---|---|
| 0.05 ng/µl Std. | 0.050 | 0.052 | 0.052 |
| 0.5 ng/µl Std. | 0.510 | 0.528 | 0.516 |
| 6 ng/µl Std. | 6.26 | 6.40 | 6.25 |
| Sample 1 | 5.54 | 5.82 | 5.48 |
| Sample 2 | 10.00 | 10.80 | 11.09 |
| Units: ng/µl | |||
Table 2. EzQuant RNA HS Kit Performance
| Sample / Expected Conc. | EzCube | Qubit™ 4 | DS-11 FX+ |
|---|---|---|---|
| 0.5 ng/µl Std. | 0.583 | 0.532 | 0.518 |
| 1 ng/µl Std. | 1.13 | 1.11 | 1.11 |
| 5 ng/µl Std. | 5.01 | 5.10 | 5.04 |
| Sample 1 | 9.25 | 9.38 | 8.99 |
| Sample 2 | 78 | 75 | Too high |
| Units: ng/µl | |||
Table 3. EzQuant ssDNA Kit Performance
| Sample / Expected Conc. | EzCube | Qubit™ 4 | DS-11 FX+ |
|---|---|---|---|
| 0.05 ng/µl Std. | 0.038 | 0.036 | 0.034 |
| 1 ng/µl Std. | 0.592 | 0.516 | 0.470 |
| 10 ng/µl Std. | 8.16 | 8.16 | 6.45 |
| Sample 1 | 0.560 | 0.592 | 0.552 |
| Sample 2 | 5.25 | 5.02 | 4.71 |
| Units: ng/µl | |||
Conclusion
Based on the experimental data, EzQuant Quantification Assay Kits deliver highly sensitive and precise results for dsDNA, RNA, and ssDNA. The EzCube fluorometer yields accurate and consistent data even with low-concentration extracted samples, performing on par with leading platforms. This high level of correlation with industry standards confirms EzCube as a reliable and robust solution for critical genomic research and diagnostic workflows.
Find out more at EzCube Fluorometer / EzQuant Quantification Assay Kits
Note:
Qubit™ and Thermo Fisher® are registered trademarks of Thermo Fisher Scientific, Inc. and its subsidiaries. DeNovix® is a registered trademark of DeNovix Inc. These trademarks are used herein for identification and reference purposes only.
The data presented in this document was obtained under specific laboratory conditions and is provided for reference only. Users are solely responsible for evaluating these results and determining their suitability for specific applications. Users assume all risks associated with the use of the data contained in this document.
