Application note from WellGenetics Inc., Taiwan, by Dr. Yawen Chen
CRISPR/Cas9 is a gene editing tool that can manipulate gene expression in plants, humans and animals. Compared to other existing genome editing methods, it is also faster, cheaper, more accurate, and more efficient. The CRISPR/Cas9 system is widely utilized in multiple areas of gene study at present.
In the workflow of CRISPR/Cas9 system, PCR screening is usually recommended to validate whether the gene editing is successful or not.
PCR preparation includes a complicated and subtle pipetting process. It always takes a lot of time and human error also occurs frequently. Using EzMate 401 automated pipetting system with CoolBlock adapters can not only save more manpower and time, but also improve the accuracy and precision by avoiding the human errors.
Figure 1. EzMate 401 in the lab of WellGenetics Inc.
Figure 2 (Left). The worktable of EzMate 401
Figure 3 (Right). The interface of software of EzMate 401
The result of the PCR is confirmed by gel electrophoresis in Figure 4. We can see the significant bands in the positive control and no bands in the negative control in the control group to prove that the result is credible. Moreover, in the experimental group, we can also easily determine which sample has the positive result. This is ideal gel electrophoresis data by using EzMate 401 automated pipetting system with CoolBlock adapters which is a great improvement on the accuracy of PCR experiments.
Figure 4. The PCR result in gel electrophoresis by EzMate 401
EzMate automated pipetting system is the best solution to handle the complicated and repeated pipetting process in the experiments of PCR preparation. It not only provides great reproducibility and repeatability of the experiment, but also easily solves the short-handed problem in lab. This system certainly makes time and manpower more efficient and also enhances the quality of eperimental data.